TRIzol reagent (Invitrogen) was used to isolate total RNA from WI-38 cells and serum samples. A PrimeScript RT reagent Kit (TaKaRa, Dalian, China) was used to generate complementary DNA (cDNA). qRT-PCR was performed using SYBR Green qPCR SuperMix (Invitrogen). Primers purchased from TaKaRa are listed in Table Table1.1. The PCR amplification programme was as follows: 94 °C for 10 min, followed by 40 cycles at 94 °C for 15 s, 56 °C for 30 s, 72 °C for 1 min, and 72 °C for 10 min. Relative expression levels of KCNQ1OT1, FOXM1, and miR-370-3p were calculated using the 2−ΔΔCt method. KCNQ1OT1 and FOXM1 were normalised to β-actin, and miR-370-3p was normalised to U6.

Primers for real-time polymerase chain reaction (qRT-PCR) in present study

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