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2.2. Micro-Raman Spectra
This protocol is extracted from research article:
Magneto-Primed Triticale Seeds Studied by Micro-Raman Spectra
Plants (Basel), May 27, 2021; DOI: 10.3390/plants10061083

A Renishaw Raman Microscope System RM2000 (United Kingdom) equipped with a Leica microscope and a 633 nm He–Ne laser Renishaw RM 2000 was used. The spectra were recorded with a 25 mW laser power. The typical spectra from 100 to 4000 cm−1 were recorded with a resolution of 4 cm−1. The time acquisition was 10 s and 5 scans were recorded to improve the signal-to-noise ratio. Correct calibration of the instrument was verified by measuring the Stokes and anti-Stokes bands and checking the position of the Si band at ±520.6 cm−1.

Ten Triticale seeds of each treatment were cut through the demarcation line using a cutter knife and placed in the microscope of the Raman device, regardless of the treatment applied. Seeds belonging to treatments C and D were dried previously with filter paper after 5 h soaking in water. From this point on, Raman spectra were obtained from both the exterior and interior of the seeds. In the interior, six points of study were selected to analyse the germ and endosperm in each lot of seeds. An examination of the seed pericarp was also conducted in the same way.

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