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RNA immunoprecipitation (RIP) assay
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RIP assays were performed using the Imprint® RNA Immunoprecipitation Kit (Sigma-Aldrich) as per manufacturer's instructions. Briefly, 1 × 106 cells were lysed with RIP buffer and then incubated overnight with antibody-coated magnetic beads at 4 °C. Anti-immunoglobulin G (Anti-IgG) served as the reference for anti-argonaute-2 (Anti-Ago2). After RNA was isolated from the magnetic beads, qRT-PCR was conducted to analyze circRANBP17 and miR-635 expression levels.
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