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THP‐1 macrophages were fixed with 4% paraformaldehyde for 30 minutes. Then, cells were rinsed twice with PBS and stained with filtered Oil red O solution (Sigma‐Aldrich) at room temperature for 15 minutes, followed by destaining with 60% isopropyl alcohol for 5 seconds. Oil red O staining was observed under a light microscope, and the intensity was measured by ImageJ software (National Institutes of Health, Bethesda, MD).
Copyright and License information: ©2021 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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