The amino acid content of the pressure-treated P. brevitarsis seulensis was measured with an L-8800 amino acid analyzer (Hitachi, Tokyo, Japan) with an ion exchange resin column (Kim et al., 2020b). The standard amino acid contents were procured from Sigma-Aldrich (St. Louis, MO, USA). The EAAI was calculated according to FAO/WHO/UNU (1985).
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