Cell Lines and Cell Culture Experiments

VW Veronika Weyerer
PS Pamela L. Strissel
CS Christine Stöhr
ME Markus Eckstein
SW Sven Wach
HT Helge Taubert
LB Lisa Brandl
CG Carol I. Geppert
BW Bernd Wullich
HC Holger Cynis
MB Matthias W. Beckmann
BS Barbara Seliger
AH Arndt Hartmann
RS Reiner Strick
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Eleven ccRCC cell lines (MZ1257RC, Caki1RC, MZ1790RC, MZ1795RC, MZ2861RC, MZ1846RC, MZ2733RC, MZ1851RC, MZ1774RC, MZ1973RC, MZ2905RC) were previously established from primary ccRCC and cultured according to Seliger et al. (32). The MZ1257RC cell line was used in all functional studies and cultured in DMEM media, 10% FCS and L-Glutamine. The human embryonic kidney cell line HEK293 was cultured in DMEM F12 media and 10% FCS. All cell lines were cultured at 37°C at 5% CO2.

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