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The construction of nude mouse tumor formation model

WZ Wei Zhu
FG Fei Gao
HZ Hongyi Zhou
KJ Ke Jin
JS Jianfeng Shao
ZX Zhuoqun Xu
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The animal study was approved by Ethics Committee of the Wuxi People’s Hospital Affiliated to Nanjing Medical University.T24 cells transfected with shCtrl or shMCM8 lentiviruses were subcutaneously injected into the four-week-old female BALB-c nude mice from Shanghai Lingchang Biological Technology Co., Ltd. (Shanghai, China) to construct the Xenograft models, each group containing five mice. During the feeding period, the tumor volume was measured. On the last day of feeding, 0.7% sodium pentobarbital was injected intraperitoneally for several min, and the fluorescence was observed by the in vivo imaging system (IVIS Spectrum, Perkin Elmer). After 40 days, the mice were sacrificed using cervical dislocation to collect tumors. The tumors were weighed and photographed, and finally frozen in liquid nitrogen and stored at − 80 °C.

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