Suite2p is a software package for the analysis of calcium imaging data [17]. We tested Suite2P on simulated datasets using the software available on github https://github.com/MouseLand/suite2p. To obtain good spatial footprints, we set the overlapping parameter to be True across simulations. When the spike amplitude was lower or equal to 0.01, we turned off the sparse_mode and when the spike amplitude was 0.005, we turned off the connected parameter. We flipped and high-passed the signals with a 15Hz filter and applied a manual threshold to extract spikes.
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