Stability of [177Lu]Lu-FAPI-04 & [177Lu]Lu-FAPI-46 in final formulations
This protocol is extracted from research article:
Fully-automated synthesis of 177Lu labelled FAPI derivatives on the module modular lab-Eazy
EJNMMI Radiopharm Chem, Apr 16, 2021; DOI: 10.1186/s41181-021-00130-3

The stability of [177Lu]Lu-FAPI derivatives were monitored for 4 days from the end of synthesis (T0) in room conditions 24 °C (Figs. 6 and and7).7). Radiochemical purity analyzes were performed by R-HPLC and R-TLC using the same methods indicated in Figs. Figs.44 and and55.

HPLC UV chromatograms of (a) FAPI-04 precursor (UV-254 nm), (b) FAPI-46 precursor (UV-264 nm) on a ACE-3 C18 150 × 3.0 mm column, mobile phase: %13 ACN / %87 Water (% 0,1 TFA), isocratic flow: 0.6 ml/min., FAPI-04 RT: 3.5–4.5 min., FAPI-46 RT: 5–6 min

R-HPLC chromatograms of (a) Free [177Lu] Lu, (b) [177Lu]Lu-FAPI-04, (c) [177Lu]Lu-FAPI-46. Free [177Lu] Lu RT: 2–3 min., [177Lu]Lu-FAPI-04 RT: 4–5 min., [177Lu]Lu-FAPI-46 RT: 6–7 min. (Method parameters are the same as depicted in Fig. Fig.2).2). R-TLC chromatograms of (d) Free [177Lu] Lu, (e) [177Lu]Lu-FAPI-04, (f) [177Lu]Lu-FAPI-46. TLC plate: ITLC SG, mobile phase: 0.05 M Citrate buffer pH 4, Free [177Lu] Lu RF: 0.8–1.0, [177Lu]Lu-FAPI-04 & [177Lu]Lu-FAPI-46. RF: 0.0–0.2

Stability study of [177Lu]Lu-FAPI-04. a Radiochemical purity results analyzed by R-HPLC: T0, T0 + 2 h, T0 + 4 h, T0 + 6 h, T0 + 8 h, T0 + 24 h, T0 + 48 h, T0 + 72 h, T0 + 96 h. b Radiochemical purity results analyzed by R-TLC: T0, T0 + 24 h, T0 + 48 h, T0 + 72 h, T0 + 96 h. (T0 = End of the synthesis)

Stability study of [177Lu]Lu-FAPI-46. a Radiochemical purity results analyzed by R-HPLC: T0, T0 + 2 h, T0 + 4 h, T0 + 6 h, T0 + 8 h, T0 + 24 h, T0 + 48 h, T0 + 72 h, T0 + 96 h., b Radiochemical purity results analyzed by R-TLC: T0, T0 + 24 h, T0 + 48 h, T0 + 72 h, T0 + 96 h. (T0 = End of the synthesis)

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.