Based on the median expression value of the real hub gene, CCA samples of the TCGA-CHOL data set were divided into low- or high-expression groups. To explore potential functions of the real hub gene, gene set enrichment analysis (GSEA) was performed to detect which KEGG pathways were enriched (24). Terms with FDR < 0.05 were visualized by R package “ggplot2” (https://cloud.r-project.org/web/packages/ggplot2/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.