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Levels of stored triglycerides in differentiated mature adipocytes were verified by staining intracellular lipid droplets with Oil red O (Solarbio) subjected to staining procedures as described previously (Oh et al., 2019). Images were taken using Olympus IX73 Inverted Microscope. To quantify the lipid content, Oil Red O was extracted with 100% isopropanol and calculated by the measuring absorbance at 490 nm using a SpectraMax i3x microplate reader (Molecular Devices, San Jose, CA, United States).
Copyright and License information: ©2021 Zhang, Sun, Liu, Lv, Hou, Lin, Xu, Zhao, Gai, Zhao and Yuan.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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