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NanoString nCounter

KP Kyrie Pappas
TM Tiphaine C. Martin
AW Andrew L. Wolfe
CN Christie B. Nguyen
TS Tao Su
JJ Jian Jin
HH Hanina Hibshoosh
RP Ramon Parsons
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Breast biopsies maximized for epithelial content were used to prepare RNA for Nanostring experiments. NanoString experiments were performed by the NanoString core at Icahn School of Medicine at Mount Sinai using probes for each gene pre-designed and validated by Nanostring. Total counts for each probe were obtained, and were normalized to the housekeeping genes (geometric mean of probe counts for all housekeeping genes) for each sample. See Supplementary Data 1 for NanoString probeset. The PTEN probes were located in the 3′UTR of PTEN because it contains unique regions from the PTEN pseudogene, PTENP1.

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