Lentivirus production and astrocyte infection

JS Juliane Schiweck
KM Kai Murk
JL Julia Ledderose
AM Agnieszka Münster-Wandowski
MO Marta Ornaghi
IV Imre Vida
BE Britta J. Eickholt
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Probes of fluorescent RAB8A and paxillin were expressed in astrocytes via lentiviral transduction. FIV particles were produced in the HEK293TN producer cell line (BioCat) and harvested analogously to HIV particles77. In all, 70% confluent astrocytes from WT and Dbn−/− mice on glass-bottom dishes were transduced 7 days before scratch wounding and live-cell imaging.

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