Cells were transduced with lentivirus vectors expressing shTNIK (Mission Lentiviral Transduction Particle, clone 33 [#1, TRCN0000234733] and clone 35 [#2, TRCN0000234735], MilliporeSigma) or nontargeting control shRNA (MISSION Non-Target shRNA Control Transduction Particles [SHC002V], MilliporeSigma). The infected cells were selected with 2 μg/mL puromycin (Thermo Fisher Scientific) for 7 days and used for further analyses.
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