4.1. Strains and Media

E. coli strain MG1655 (F− λ− ilvG rfb-50 rph-1) [58] carrying pETgfp-mut2-AGGAGG(3) plasmid [59] was used. Bacteria were grown in LB-Miller media and in MOPS (morpholinepropanesulfonic acid) media supplemented with 0.1% glucose (MOPS-Glc), as described [60]. All growth media were supplemented with kanamycin to a final concentration of 25 µg/mL. For overexpression experiments, MG1655 bacteria with and without pBbE6k plasmids [61] were used. Media was supplemented with IPTG (Isopropyl β-d-1-thiogalactopyranoside) (Roth. Germany) to a final concentration of 1 mM.