Preculture

FS Felix Schottroff
JK Jens Kastenhofer
OS Oliver Spadiut
HJ Henry Jaeger
DW David J. Wurm
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Preculture medium (500 ml) was inoculated with 1 ml of a frozen cell stock in a baffled shake flask. The preculture was incubated overnight at 37°C and 200 rpm in a shaking incubator (Infors AG, Bottmingen, Switzerland).

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