Sequencing reads were trimmed using Trimmomatic v0.33 (Bolger et al., 2014) and aligned to mm10 assembly using bwa-mem v0.7.17 (Li, 2013). PCR duplicate sequencing reads were removed using MarkDuplicates from Picard toolkit (http://broadinstitute.github.io/picard/). Broad peak calling on the de-duplicated BAM files was performed using MACS v2.1.2 (Zhang et al., 2008).
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