U251 cells were seeded into 96-well plates at a density of 5×105 cells/well and cultured for 24 h, followed by continuous culture with PSH for 72 h at 37°C. Cell viability was examined by CCK-8 assay. Briefly, after the treatments, 10 µl CCK-8 solution was added into each well and incubated at 37°C for 4 h, and the absorbance was measured at 455 nm. The cells in the control group were cultured in normal medium. Data are presented as the fold-change relative to the control group.

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