Wild type and LOG OX1 cells were collected at two time points: 5 h after the dark phase and 1 h before the dark phase, each with three biological replicates, and this experiment was repeated three times. These cells were pelleted down at 5,000 × g for 5 min, and the supernatant was discarded. The pellet was washed with 1 ml of 1 × PBS (pH 7.6), and finally, the pellet was resuspended in 1 ml of 70% ethanol and stored at 4°C until further use. For staining, the 70% ethanol was removed. The pellet was washed with 1 × TBS (pH 7.6) and stained with 1 μl of working concentration of DAPI (Sigma, United States) in 1 ml of 1 × TBS for 30 min at room temperature, which was followed by flow cytometric analysis in the Aria II Flow cytometer (BD Biosciences) as per manufacturer’s protocol.

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