Wild-type human PATL2 were constructed and then recombined with the eukaryotic expression vector pcDNA3.1. A MYC-tag or a FLAG-tag were fused at N-terminus of PATL2. The vectors were constructed by GenScript (Nanjing). The variant c.649T>A was generated using Fast Mutagenesis Kit (Vazyme, Nanjing, C214). GFP reporter plasmid for analyses of Mos 3′-UTR activities were constructed by GenScript (Nanjing) (Dai et al., 2019). The PATL2WT-MYC and PATL2Y217N-MYC plasmids were linearized with XbaI enzyme (New England Biolabs, #R0145V) and then be transcribed to PATL2WT and PATL2Y27N cRNAs using HiScribe T7 ARCA mRNA Kit (New England Biolabs, E2060) according to the manufacturer's standard mRNA synthesis protocols. Mos 3′-UTR reporter plasmid was linearized and in vitro-transcribed using the SP6 mMESSAGE mMACHINE Kit (Invitrogen, AM1340) according to the manufacturer's instructions.

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