The sterilized scaffolds were placed in a 24-well plate under sterile conditions. Then, they were washed 3 times with phosphate-buffered saline (PBS; Boster, pyg0021, Wuhan, China) and bathed in the α-MEM medium without FBS for 2 h before cells seeding. Then, 6 × 104 BMSCs were seeded into a 24-well plate in which a scaffold was ensconced. After the cells adhered to the scaffolds completely, the cell-seeded scaffolds were transferred to a new 24-well plate for further culture. Immediately after, these cell-scaffolds were randomly divided into two groups. The group treated with EMF was presented as the EMF group, while the group without any intervention was regarded as the control group (Ctrl group).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.