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To eliminate the animal sickness factors and avoid the biases due to sickness and blunted behaviors induced by LPS, OFT was performed according to the previously developed protocols [50]. Briefly, mice were adapted to the experimental room for 1 h and were placed in the chamber of 45× 45 × 30 cm. A total of a 15-min video was recorded to observed the mice locomotor activity. The total distance covered by mice was measured, analyzed, and expressed in meters.
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