Total mRNA Isolation and Real-Time PCR Analysis

Total mRNA of MRC-5 cells was extracted using RNA extraction kit (Vazyme) and the extracted mRNA was measured by using Nanodrop spectrophotometry (Thermo-Scientific, Wilmington, DE). Total mRNA was reverse-transcribed, and cDNA was then subjected to real-time PCR analysis. The levels of MYH9 and GAPDH mRNA expression were measured by using the SYBR Green kit (Thermo). These primers’ sequences were as follows: MYH9: sense, 5ʹ- CCTCAAGGAGC- GTTACTACTCA-3ʹ; antisense, 5ʹ-CTG​TAG​GCG​GTG​TCT​GTG​AT-3ʹ, and GAPDH: sense, 5ʹ-GCT​GGC​GCT​GAG​TAC​GTC​GTG​GAG​T-3ʹ; antisense, 5ʹ-CAC​AGT​CTT-CTG​GGT​GGC​AGT​GAT​GG-3ʹ. These primers were acquired from Shanghai Sangon.

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