Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
The AAF/III determinant (agg3DCBA) was amplified by PCR using EAEC strain 55989 plasmid DNA as template and primer pair agg3_for/agg3_rev. The PCR product was digested with XbaI and HindIII and cloned into the HindIII/XbaI-digested pBAD24.
Copyright and License information: ©2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
