2.4.3. Proliferation Analysis

VSOPs-labeled and unlabeled hASCs were seeded in 24-well plates with a density of 2.5 × 10⁴ cells each and maintained in EM-DMEM at 37 °C with 5% CO₂ for 12 days. VSOPs concentrations of 1.5 mM and 0.15 mM were used for labeling. The proliferation analysis was performed in duplicate for each patient, each VSOPs concentration and for unlabeled cells, resulting in 10 wells per patient per day. Every second day the cells were detached with trypsin and counted with an automated cell counter (Casy^® Technologies, Innovatis AG, Reutlingen, Germany).