2.7. Analysis of Atomic Force Microscopy (AFM)

Atomic force microscopy was used to observe the possibility of L. acidophilus NCFM (109 cfu/mL) to bind B(a)P. Four groups were prepared as follows. The first group was the inorganic system which contains 10 μg/mL B(a)P and strain NCFM; the second was the organic system with 10 μg/mL B(a)P and strain NCFM; the third was 10 μg/mL B(a)P solution plus strain NCFM; and the fourth was only bacterial suspension as the control. These mixtures were incubated at 37°C for 1 hr and then diluted to a final concentration of 10 μg/mL. 5 μL was sampled and dispersed onto a mica carrier (PELCO mica disc 10 mm) for drying at room temperature. AFM images were captured by using a scanning probe microscope (NTEGRA Spectra, NT-MDT Co., Ltd., Moscow, Russia) in the tapping mode.

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