293T cells were equally seeded in 5 wells in 24-well plates (5 × 104 cells per well). Then 24 h later, the cells were treated with actinomycin D (2 μg/ml, HY-17559, MedChem Express, Monmouth Junction, NJ, USA) for 0 h, 4 h, 8 h, 12 h, and 24 h, respectively. After that, the cells were harvested, and the relative RNA levels of circ-SMO and linear SMO were analyzed by qRT-PCR and normalized to the values measured in the 0 h group.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.