293T cells were equally seeded in 5 wells in 24-well plates (5 × 104 cells per well). Then 24 h later, the cells were treated with actinomycin D (2 μg/ml, HY-17559, MedChem Express, Monmouth Junction, NJ, USA) for 0 h, 4 h, 8 h, 12 h, and 24 h, respectively. After that, the cells were harvested, and the relative RNA levels of circ-SMO and linear SMO were analyzed by qRT-PCR and normalized to the values measured in the 0 h group.

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