Mice were received intravenous administration of buffer, aptamer mixtures (20 μM, 100 μl), the mixture of origami and aptamers (Ori + H + T, aptamers 20 μM, origami ~560 nM, 100 μl) and Aptarray (~560 nM, 100 μl). Blood samples were collected at 24 h post-injection and sent to the laboratory of Pony Testing International Group (Beijing, China) for analysis of the levels of alanine transaminase (ALT), aspartate transaminase (AST), creatinine (Cr), and blood urea nitrogen (BUN). The plasma levels of TNF-α and IL-6 were analyzed by corresponding ELISA kits (Cloud-Clone Crop). Major organs (including the heart, liver, spleen, lung, and kidney) were harvested from mice for hematoxylin and eosin staining to evaluate morphological changes caused by the administration of DNA materials.

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