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At 3 mpi (aged 5–6 months), mice were placed facing upwards at the top of a ¼″ diameter round 3-foot long pole. Each mouse was timed for the duration to turn downwards down the pole as well as descend to the bottom of the pole. At 6 mpi (mouse age 8–9 months), wire footholds were added to the pole to assist mice in descending safely due to age and weight gain.
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