The wild type sequences of circNOL10 or SOCS2-3′UTR containing the miR-767-5p binding sites were amplified and inserted into dual luciferase reporter vector pmirGLO (Promega, Madison, WI, USA). The mutant version of circNOL10 or SOCS2-3′UTR in which the miR-767-5p binding sites were replaced was cloned into the same luciferase reporter. Then, BT-549 and MDA-MB-231 cells were co-transfected with the constructed luciferase reporter, pRL-TK Renilla luciferase reporter and miR-NC or miR-767-5p by using Lipofectamine 3000 (Invitrogen). At 48 h after transfection, Dual-Lucy assay kit (Solarbio, Beijing, China) was used to examine the luciferase activity. The Renilla activity was used as the internal reference.
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