The CHIP Kit (ab500, Abcam, Cambridge, UK) was used according to the manual instructions. The LN229 or T98G cells cultured in 10 cm dishes were crosslinked with 1% formaldehyde in PBS and plates were incubated on a rotator for 10 min at 25 °C. Subsequently, formaldehyde was removed and crosslinking quenched by incubation with 125 mM glycine in PBS for 5 min at 25 °C. Following solution removal, plates were chilled on ice and the cells lysed by adding 2 mL of cold lysis buffer with a protease inhibitor (04693116001, Roche, USA). The chromatin was fragmented to 200-500 bp with a Misonix S3000 Sonicator (Farmingdale, USA) at 4 °C. After centrifugation at 10,000 × g for 5 min at 4°C, chromatin supernatants were diluted with cold IP dilution buffer. The human YAP1 antibody (1 µg, ab52771, Abcam, Cambridge, UK) or human TEAD4 antibody (1 µg, ab58310, Abcam, Cambridge, UK) was added to chromatin and the mixture was incubated at 4 °C overnight. Dynabeads were added to the chromatin/antibody mixture and incubated for additional 4 h at 4 °C. Beads were washed with the wash buffer and samples were eluted with 250 µl elution buffer. The eluted samples were treated with 0.2 M NaCl and 1 mg/mL Protease K at 65 °C overnight. Chip samples were purified with phenol/chloroform and precipitated with cold ethanol and glycogen.

qPCR analysis was performed on the CFX96 Touch Real-Time PCR system (Bio-Rad, MA, USA) using a SYBR Premix (Bio-Rad, MA, USA) according to the manufacturer's instructions. The primer sequences used were as follows: NUPR1-F: 5ʹ-GATCAGCCTGTCCAACATGGTGAAAC-3ʹ; NUPR1-R: 5ʹ-TTTGAAATGGAGTCTCTGTC-3ʹ.

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