The overlay agar method referred to Abudoleh & Mahasneh [22] was used with modifications for this screening. Each Actinomycetes isolate inoculated onto YMEA with a straight streak method and incubated at 28 °C for 2 days. C. violaceum was grown separately in 50 mL of Luria-Bertani Broth (LB) (Oxoid) and incubated at 28 °C 24 h. Indicator bacteria then diluted until OD600 = 0.132, then a total of 100 μL culture were taken and mixed with semisolid agar (0.75% LA) of 5 mL volume for an overlay on top of the selected Actinomycetes bacteria in the YMEA before, later incubated at 28 °C for 2 days. The positive results were indicated by the absence of violacein pigmentation around the Actinomycetes isolates.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.