Single cells from fresh primary tumors, adjacent noncancerous tissue, and distal noncancerous tissue were obtained. After blocking with Fc Receptor Blocking Solution (Biolegend Cat #422301), cell surface staining was performed in FACS buffer containing antibody cocktails (anti‐CD45, anti‐CD38, anti‐CD19) on ice for 1 hour. For detection of total IgG, cells were stained for both surface IgG and intracellular IgG. The intracellular IgG was strained by using an Intracellular Fixation and Permeabilization Buffer Set (BD Biosciences Cat #558126) according to the manufacturer's protocols.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.