Single cells from fresh primary tumors, adjacent noncancerous tissue, and distal noncancerous tissue were obtained. After blocking with Fc Receptor Blocking Solution (Biolegend Cat #422301), cell surface staining was performed in FACS buffer containing antibody cocktails (anti‐CD45, anti‐CD38, anti‐CD19) on ice for 1 hour. For detection of total IgG, cells were stained for both surface IgG and intracellular IgG. The intracellular IgG was strained by using an Intracellular Fixation and Permeabilization Buffer Set (BD Biosciences Cat #558126) according to the manufacturer's protocols.

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