Short, 5′3P-bearing RNA molecules were obtained from the pCI-neo plasmid linearized by Xba I before transcription. T7 transcription reactions were performed with a T7 RiboMAX Express Large-Scale RNA Production System (#P1320, Promega). Poly(I:C) ligands are LMW (#tlrl-picw, Invivogen) and HMW (#tlrl-pic, Invivogen).

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