P8 pups were immobilized on a stereotactic frame using a custom head rest and sedated with either intraperitoneally administered Nembutal (40 mg/kg) or gaseous isoflurane. The skin atop the head was opened 0.5 cm from the bregma, and the needle was inserted through the skull at approximately 3.2 mm caudal by 0.7 mm lateral by 1.2 mm deep. Mice were injected into the right lateral ventricle with 5 μl of vehicle (HBSS) or 18:1 LPA using a 35- to 36-gauge needle and a glass 10-μl NanoFil syringe (World Precision Instruments). Postoperative flunixin meglumine (1 mg/kg; 0061-0851-03, Merck) was administered subcutaneously for 2 days for pain management, and mice were monitored daily until tissue harvest.

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