Western blot analysis was performed, as previously described (40). Anti-Flag antibody was purchased from Sigma-Aldrich (St. Louis, MO). Anti–β-tubulin antibody was purchased from The Developmental Studies Hybridoma Bank. Anti-PARP, anti-caspase3, anti-Tcf7l2, and anti–β-catenin antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-His antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-GLUT1 and anti-Zic2 antibodies were purchased from Abcam (Cambridge, MA).

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