The importin plasmids were either obtained directly from the human complementary DNA (cDNA) library as in pLX307 vector or cloned into pCDH vector with a C-terminal V5 tag. BIMAX2 (RRRRRRKRKREWDDDDDPPKKRRRLD) (29) and M9M (GGSYNDFGNYNNQSSNFGPMKGGNFGGRFEPYANPTKR) (30) were cloned into pEGFP-C2 vector to enable the expression of N-terminal EGFP fusion proteins. RTA-RFP was prepared by cloning of RTA into a modified pcDNA3 vector containing a C-terminal RFP sequence.

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