I-AD+ cells were sorted from spleens of UUO GFP mice and pooled into a single-cell suspension. GFP+I-AD+ splenocytes (1 × 104) were injected under the renal capsule, into the upper pole in kidneys (C57BL/6J mice) on day 3 after UUO surgery. Mice were euthanized 6 hours after injection. Renal tissue samples were fixed in 4% paraformaldehyde (PFA).

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