The main objective of this study was to characterize the activity of two modified recombinant BoNT/B in several preclinical models, including hiPSCs and a transgenic mouse with humanized Syt2 (hSyt2 mice), the major receptor for BoNT/B in skeletal muscle. The activity of the modified toxins was compared to unmodified BoNT/B1 and BoNT/A1. Last, we crystallized one of the recombinant modified BoNT/Bs to obtain mechanistic insight into how the modified BoNT/B interacts with its human receptor.

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