Specific pathogen–free (SPF) C57BL/6 J mice (#000664), Tie2-GFP mice (#003658), and Ubiquitin-Cre-ERT2 mice (#007001) were purchased from the Jackson Laboratory. Angpt2-EGFP mice (Tg [Angpt2-EGFP] DJ90Gsat/Mmcd) were purchased from the Mutant Mouse Regional Resource Centers. Angpt1-GFP (21), Tie2flox/flox (22), and Angpt1flox/flox (27) mice were transferred and bred in our SPF animal facilities at Korea Advanced Institute of Science and Technology (KAIST). VE-cadherin-Cre-ERT2 mice (23) were mated with Tie2flox/flox mice to obtain EC-specific Tie2-deleted mice in a tamoxifen-dependent manner. To deplete Angpt1 globally, Angpt1flox/flox mice were crossed with Ubiquitin-Cre-ERT2 mice. Two milligrams of tamoxifen (T5648, Sigma-Aldrich) was injected intraperitoneally a total of three times every 2 days to 8-week-old genetically modified mice. All mice were fed with free access to a standard diet (PMI LabDiet) and water. All mice were anesthetized by an intramuscular injection of ketamine (40 mg/kg) and xylazine (12 mg/kg) before any procedures. All animal care and experimental procedures were carried out in accordance with the protocol approved by the Institutional Animal Care and Use Committee of KAIST (no. KA2015-15), and mice were handled according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research.

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