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After complete workup the amount of amino acids in hydrolysates was determined by ninhydrin assay and referenced to a calibration of l-leucine concentrated between 5 and 100 µM as described previously9. The absorbance was determined at 546 nm with an Infinite M200 microplate reader (Tecan, Männedorf, Switzerland) using 96-well plates. Each sample was prepared three times.
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