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The lentiviral pgRNA-expressing vector was constructed by cloning the human U6 promoter, ccdB cassette and gRNA scaffold into pLL3.7 (Addgene, Inc.) by replacing its original U6 promoter8. The scaffold-linker-U6 fragment was cloned into pEASY-Blunt plasmid (TransGen Biotech).
Copyright and License information: ©2016
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