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Succinate dehydrogenase (SDH) staining
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Transverse EDL muscle sections were incubated for 3 min at room temperature in a sodium phosphate buffer containing 75 mM sodium succinate (Sigma), 1.1 mM Nitroblue Tetrazolium (Sigma) and 1.03 mM Phenazine Methosulphate (Sigma). Samples were then fixed in 10% formal-calcium and cleared in xylene prior to mounting with DPX mounting medium (Fisher). Densitometry of the samples was performed on a Zeiss Axioskop2 microscope mounted with an Axiocam HRc camera. Axiovision Rel. 4.8 software was used to capture the images.
Copyright and License information: ©2016, Omairi et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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