Construction of stable cell lines with HPV-16 E7 overexpression and MAOA knockout

CRISPR/Cas9 technology was performed to knock out MAOA in the stable HPV-16 E7-overexpressing NSCLC cells to establish stable cell lines with HPV-16 E7 overexpression and MAOA knockout (abbreviation: 16 E7-MAOA KO). A lentivirus vector (U6-sgRNA-EFS-Cas9-P2A-Puro) was constructed by Shanghai Fubaiao Biotechnology Co., Ltd. To obtain 16 E7-MAOA KO cells, the infected cells were screened by incubation with 2 μg/ml of puromycin. The efficiency of MAOA knockout was analyzed by Western blotting.