Primer Design

A total of 33 different enzymes involved in carbon metabolism pathways of the diatom P. tricornutum were selected and the corresponding genes (74) coding each enzyme (see Figure 6 and Supplemental Data 1) were searched in genomic data published by Kroth et al. (2008).

Cellular pathways and processes of central carbon metabolism in Phaeodactylum tricornutum. All red depicted enzymes are the key enzymes and their related gene/isogenes are listed beside. RUBP, D-ribulose-1,5-bisphosphate; 3PG, 3-phospho-D-glycerate; BPG, 1,3-diphosphateglycerate; GAP, D-glyceraldehyde-3-phosphate; DHAP, dihydroxyacetone phosphate; FBAC, chloroplastic fructose-1,6-bisphosphate aldolase; FBP, fructose-1,6-bisphosphate; F6P, D-fructose-6-phosphate; X5P, D-xylulose-5-phosphate; Ru5P, D-ribulose-5-phosphate; SBP, D-sedoheptulose-1,7-bisphosphate; S7P, D-sedoheptulose-7-phosphate; R5P, D-ribose-5-phosphate; E4P, D-erythrose-4-phosphate; 2PGL, 2-phosphoglycolate; 2PG, 2-phosphoglycerate; PEP, phosphoenolpyruvate; OAA, oxaloacetate; A-CoA, acetyl-CoA; G1P, D-glucose 1-phosphate; G6P, D-glucose-6-phosphate; GNL6P, D-glucono-δ-lactone-6-phosphate; 6PGN, 6-phospho-D-gluconate; PPS, periplasmic space; ER, endoplasmic reticulum (chloroplastic); ACC1, ACC2, Acetyl-CoA-carboxylase; PDH1, PDHA1, PDHB, pyruvate dehydrogenase E1; BGS1, glycosyl transferase.