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Cytospin analysis

MK Matthew Ku
RM Ruth N. MacKinnon
MW Meaghan Wall
NN Nisha Narayan
CW Carl Walkley
HC Heung-Chin Cheng
LC Lynda J. Campbell
LP Louise E. Purton
HN Harshal Nandurkar
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The cytospin was performed using a Shandon Cytospin 2 cytocentrifuge. Labeled slides were inserted into the cytocentrifuge chambers. Cell suspensions were then made from the methylcellulose colonies selected using 200 ul of FACS buffer (PBS + 2% FCS). The cell suspensions were added to the cytocentrifuge, and spun at 600 rpm for 10 minutes. The cells were then transferred to the slides in the chambers. The cytospin slides were removed from the cytocentrifuge and allowed to dry. They were stained with hemotoxylin and eosin and analyzed by light microscopy with regards to their morphology.

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