C. elegans strains

The following strains were used vs.: wild-type N2 Bristol, VP596 vsIs33[dop-3p::DsRed2];dvIs19[gst-4p::GFP], VP604 kbIs24[gpdh-1p::DsRed2;myo-2p::GFP;unc-119 rescue], SJ4005 zcIS4[hsp-4p::GFP], SJ4100 zcIs13[hsp-6p::GFP], QV65 gpIs1[hsp-16.2p::GFP];vsls33[dop-3p::DsRed2], QV285 frIs7[nlp-29p::GFP, col-12p::DsRed], IG274 frIs7, CB128 dpy-10(e128), CB88 dpy-7(e88), BE3 sqt-2(sc3), CB61 dpy-5(e61), QV41 dpy-10(e128);vsls33;dvIs19, QV248 dpy-7(e88);dvIs19, QV261 dpy-7(e88);kbIs24, IG1689 dpy-7(e88);frIs7, IG1710 dpy-7(e88);elt-3(gk121);frIs7, IG1705 dpy-7(e88);sta-2(ok1860);frIs7, IG1685 dpy-3(e27);frIs7, G1709 dpy-3(e27)elt-3(gk121);frIs7, IG1704 dpy-3(e27);sta-2(ok1860);frIs7, IG1457 dpy-10(e128);frIs7, IG1712 dpy-10(e128);elt-3(gk121);frIs7, IG1707 dpy-10(e128);sta-2(ok1860);frIs7, TP12 kaIs12[COL-19::GFP], VP332 gpdh-1(kb24);gpdh-2(kb33), LD001 ldIs7[skn-1B/C::GFP + pRF4(rol-6(su1006))], and GR2245 skn-1(mg570). QV251, which contains a reporter for gst-10p, was generated using PCR to fuse 951-bp upstream from the start codon to GFP (Hobert 2002). The fusion PCR product was then injected at ∼20 ng/μl with myo-3p::dsRed as a comarker. Unless noted otherwise, worms were cultured at 20° using standard methods (Brenner 1974).