4.4 SureSelect RNA target enrichment: library preparation, hybridisation and enrichment

In the SureSelect RNA sequencing runs, all RNA samples were dried by vacuum centrifugation before resuspension using 19 μl RNASeq Fragmentation Mix. Fragmentation, generation of adapter-ligated cDNA libraries, hybridisation, PCR and all post-reaction clean-up steps were performed according to the SureSelectXT Automated RNA Target Enrichment protocol. All recommended quality steps were performed.