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Virus neutralization assays were performed on the TZM-bl cell line, using replication-competent or pseudotyped viruses grown in human PBMC as previously described (67). Serial dilutions of serum samples were tested for neutralization of a panel of tier 1 (SHIV-SF162P4 and SHIV-1157iEL-p [replication-competent viruses]) and tier 2 (SHIV-SF162P3.5 and SHIV-89.6P.18 [pseudotyped viruses] as well as SHIV-89.6 and SHIV-1157ipd3N4 [replication-competent viruses]) SHIVs in TZM-bl cells.
Copyright and License information: ©2017 Shen et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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