Morphometric Analysis

For morphometric analysis, data from three independent replicates with separate cohorts of zebrafish embryos were analyzed for the main effects of treatment. All measurements were performed using ImageJ software. Width of somitic jam2a expression was measured as a fraction of the length of the domain of expression along the medio-lateral axis (denoted y), divided by length of the domain of expression along the anterior-posterior axis (denoted x) of the eighth jam2a-expressing somite on the right side of the embryo (fraction y/x). Notably, the length of the domain of jam2a expression along the anterior-posterior axis (x) of the eighth jam2a-expressing somite did not significantly differ in control (DMSO) versus DEAB-treated embryos (P ≥ 0.2624).